Viscosity measurements were do
Viscosity measurements were done on native form of Protein XYZ and on 4
denaturants. The crude Protein XYZ was diluted to produce 5% w/v. Using the
Ostwald viscometer, the following data were obtained:
time, sec | time, sec | time, sec | time, sec | |
Denaturant | Blank (Native) |
Native | Blank (Denaturant) |
Denatured |
pH= 2.00 | 70 | 95 | 70 | 101 |
Temp (90 °C) | 70 | 98 | 70 | 150 |
0.1M Urea | 72 | 97 | 88 | 110 |
DTT | 71 | 96 | 90 | 170 |
a. Calculate and tabulate the reduced viscosities (in mL/g) of the native and
denatured Protein XYZ.
b. Determine two most effective denaturants.
c. What can be inferred with the two most effective denaturants?
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